Health
A new look inside Ebola’s “viral factories”
Two views of Ebola’s “viral factories” inside host cells. The image on the left was captured via confocal immunofluorescence microscopy and shows Ebola’s viral factories in pink. The image on the right was captured using electron tomography and shows viral factories in orange.
Newswise — LA JOLLA, CA—New research in the journal Nature Communications gives scientists an important window into how Ebola virus replicates inside host cells. The study, led by scientists at La Jolla Institute for Immunology (LJI), reveals the inner workings of “viral factories,” clusters of viral proteins and genomes that form in host cells.
The research team, which included experts from Scripps Research and UC San Diego School of Medicine, found that Ebola virus’s replication machinery forms fascinating microscopic structures that become viral factories. By understanding the architecture and function of these microscopic manufacturing hubs, researchers may be closer to developing new therapies that interrupt the Ebola virus life cycle and prevent severe disease.
“We are imaging these fluid and dynamic assembly centers for the first time. Understanding how they work and what they require gives us the information needed to defeat them,” says LJI President and CEO Erica Ollmann Saphire, Ph.D., senior author of the new study.
What is a viral factory?
Scientists first spotted what would turn out to be “virus factories” in virus-infected animal cells back in the 1960s, but they didn’t know what they were seeing. Within a sea of normal cellular proteins, these areas looked like fuzzy splotches.
“People had already seen that Ebola-infected cells had these ‘inclusions,’” says LJI Postdoctoral Researcher Jingru Fang, Ph.D., first author of the new study. For a long time, scientists thought of these “inclusions” as helpful visual indicators of infection, without understanding their true purpose. “But in fact, these ‘inclusion bodies’ actively gather an enormous quantity of viral proteins and viral RNAs.”
Many viral pathogens, including rabies virus and RSV (respiratory syncytial virus) form inclusions in host cells, Fang explains. “Recent studies suggest that these cellular inclusions are the site where viruses make their RNA genomes. They are ‘viral factories’ with actual functional purpose: to offer a secured space for viral RNA synthesis,” says Fang. “The process of viral RNA synthesis involves flux of viral building blocks. This means molecules gathered inside viral factories should be able to move freely rather than being static.”
For the new study, Saphire, Fang and their colleagues wondered: Can we observe the movement of viral building blocks directly in living cells?
Fang began by tagging a viral protein called VP35 with a fluorescent marker that makes the protein glow in the dark. VP35 is a critical component of the viral factory and is important for viral RNA synthesis (and the making of new copies of Ebola virus). Working with imaging experts in the LJI Microscopy and Histology Core, Fang followed the glowing proteins in live cells, which express a simplified and non-infectious version of Ebola viral factories.
Under the microscope, Fang and colleagues could indeed see and even measure how molecules move inside the viral factories formed in host cells. This finding added evidence that viral proteins are clumping together like droplets so they can churn out the proteins needed to help the virus replicate. Those mysterious inclusions really are viral factories. The researcher dubbed these “droplet-like” viral factories.
Then the scientists saw something odd. Some of the glowing proteins didn’t gather into clumps. Instead, they joined up with a smattering of other viral proteins, creating a fluorescent swirl that evoked van Gogh’s “Starry Night.” These trails of viral proteins still had the right ingredients to replicate Ebola virus, so the scientists dubbed them “network-like” viral factories.
“These are two different flavors of the viral factory,” says Fang. “People have mostly focused on the droplet-like form, which is the majority, and not paid too much attention to this other form.”
Besides their shapes, there was a key difference between the two factories. It appeared the network-like factories had the right ingredients for the incoming Ebola virus to express its genes, but they didn’t actually produce virus progenies.
A multi-tasking machine
Next, the researchers looked at a key player in infection: a protein called virus polymerase. Polymerase is a multifunctional nanomachine that comes with the virus. This machine not only copies the Ebola virus genomic material, it also transcribes the viral genome into messenger RNAs, which instruct infected cells to produce loads of viral proteins. The researchers wanted to understand how this viral machine functions inside viral factories.
Ebola virus polymerase is already known as a hard-working protein—all Ebola viral proteins have to be. Ebola virus is a highly efficient pathogen because it gets by with just seven genes (humans have more than 20,000 genes). Saphire has led research showing that Ebola virus survives by making proteins that can transform and take on different jobs during the course of infection.
Just last year, Saphire, Fang, and collaborators published a related discovery that viral polymerase actually harnesses a druggable human protein to help the virus replicate its genome. The team reported that while polymerase is essential for viral replication, the polymerase doesn’t actually jump into action until infection is well underway.
This work was important for understanding how polymerase stepped into action, but scientists also needed to know where polymerase was active. Fang knew it would be important to look at what polymerase might be up to in viral factories.
The researchers discovered that polymerase actually builds its own special structures inside viral factories. Many copies of polymerase gather in small bundles, called foci. The researchers found that these bundles spread out when a droplet-like viral factory starts replicating viral material.
Scientists aren’t sure exactly why polymerase needs to form bundles before it can do its job, but the spatial arrangement of the bundles must be important. As Fang points out, the idea of many small components coming together to build a structure isn’t a new concept in nature. “You can use a beehive or coral reef as the analogy to help understand why a specific spatial arrangement is important for a biological system to function,” she says.
With this finding, scientists now know how to find different kinds of viral factories and how polymerase organizes itself down on the factory floor.
Fighting back
More than 30 human pathogens are known to assemble viral factories inside host cells, including respiratory syncytial virus (RSV) and even rabies virus. With this new view of Ebola’s viral factories, the scientists are curious whether other viruses construct similar forms of viral factories—and whether other viruses use their own versions of polymerase in the same way.
“If that’s true, maybe we can target the feature of viral factory formation that has been shared by multiple different viruses,” says Fang.
Going forward, Fang would also like to study how Ebola virus forms viral factories in different kinds of host cells. Do these viral factories look different in cells from animals (such as the virus’s natural hosts, the fruit bats) that can carry the virus around without getting sick? “Can we find some explanation for host-specific viral pathogenesis?” she asks.
The new study also demonstrates the importance of collaboration across San Diego’s Torrey Pines Mesa. The LJI team worked closely with Scripps Research Professor Ashok Deniz, Ph.D., and UC San Diego Professor Mark H. Ellisman, Ph.D., Director of the National Center for Microscopy and Imaging Research.
“The combination of state-of-the-art tools available on the Torrey Pines Mesa allowed us to combine the biophysical characterization with the human health insight,” says Saphire
Additional authors of the study, “Spatial and functional arrangement of Ebola virus polymerase inside phase-separated viral factories,” include Guillaume Castillon, Sebastien Phan, Sara McArdle, Chitra Hariharan, and Aiyana Adams.
This study was supported by the National Institute of Health (grants NIH S10OD021831, R24GM137200, and S10OD021784), an Imaging Scientist grant (2019‐198153) from the Chan Zuckerberg Initiative, LJI institutional funds, and the Donald E. and Delia B. Baxter Foundation Fellowship.
DOI: 10.1038/s41467-023-39821-7
Source: La Jolla Institute for Immunology
About La Jolla Institute
The La Jolla Institute for Immunology is dedicated to understanding the intricacies and power of the immune system so that we may apply that knowledge to promote human health and prevent a wide range of diseases. Since its founding in 1988 as an independent, nonprofit research organization, the Institute has made numerous advances leading toward its goal: life without disease. Visit lji.org for more information.
Journal Link: Nature Communication, July-2023
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What You Don’t Know Can Hurt: 3 facts about updated COVID-19 vaccines
Despite post-pandemic reunions, updated COVID-19 vaccines remain crucial for protection, especially for high-risk groups. Mild side effects are typical, severe ones rare.
(Family Features) Four and a half years after the start of the COVID-19 pandemic, people are once again enjoying time with friends and family and doing their favorite things. However, the virus that causes COVID-19 keeps changing, so it’s still important to stay up to date on your vaccinations. Respiratory viruses, including COVID-19, are common during the fall and winter months. That’s why now is a good time to take care of yourself and your loved ones by getting the facts on the updated COVID-19 vaccines.
“As the virus changes, we are learning more and more about how to protect people from COVID-19,” said Namandjé N. Bumpus, Ph.D., principal deputy commissioner at the U.S. Food and Drug Administration (FDA). “Getting the latest COVID-19 vaccine means your protection is as up to date as possible.”
Here is information about this season’s COVID-19 vaccines from the Risk Less. Do More. public education campaign:
Vaccines are the best way to protect yourself and your loved ones from COVID-19. Getting vaccinated against COVID-19 lowers your chances of getting very sick or being hospitalized. Some people who get the vaccines still get sick, but vaccination can make symptoms milder. This is important if you are over age 65 or have certain health conditions. Both can put you at a higher risk for serious or long-lasting health problems, such as Long COVID or even death from COVID-19. If you do get COVID-19, ask your doctor about treatment options to reduce your risk of severe illness.
COVID-19 vaccines are regularly updated to keep up with the changing virus. The COVID-19 vaccines are updated based on the latest variants of the virus. The updated vaccines offer the best protection against the changing virus that causes COVID-19. Getting the latest vaccine boosts your immunity and lowers your risk of severe sickness.
Side effects for COVID-19 vaccines are usually mild and go away on their own in a few days. The most common side effects are soreness or redness at the injection site, headache or body aches, or fever. Severe side effects like anaphylaxis are rare, with about five adverse events per 1 million doses given.
Staying informed and getting this season’s COVID-19 vaccine – and encouraging loved ones to do the same – is one of the best things you can do to protect yourself and others this fall and winter. Knowing the facts about COVID-19 vaccinations can give you peace of mind and support a safer, healthier future for all. For more information, visit cdc.gov/RiskLessDoMore or talk to your doctor.
If you think you have COVID-19, testing can help. All U.S. households are now eligible to receive four free COVID-19 test kits. Order them today at COVIDTests.gov.
The Impact of COVID-19 In Black and Hispanic Communities
For the first two years of the COVID-19 pandemic, people in Black and Hispanic communities were consistently more likely to get COVID-19, need medical or hospital care, and die from COVID-19 than people in other communities. That gap has narrowed over time, but this past summer, Black people were still more likely to be hospitalized for COVID-19 compared to the average across all races and ethnicities combined. Hispanic people had lower than average rates of hospitalization. For everyone, getting the latest COVID-19 vaccine offers the best protection against severe disease and hospitalization.
Photos courtesy of Shutterstock
SOURCE:
United States Department of Health and Human Services
Our Lifestyle section on STM Daily News is a hub of inspiration and practical information, offering a range of articles that touch on various aspects of daily life. From tips on family finances to guides for maintaining health and wellness, we strive to empower our readers with knowledge and resources to enhance their lifestyles. Whether you’re seeking outdoor activity ideas, fashion trends, or travel recommendations, our lifestyle section has got you covered. Visit us today at https://stmdailynews.com/category/lifestyle/ and embark on a journey of discovery and self-improvement.
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Alvin Thomas, University of Wisconsin-Madison and Quinn Kinzer, University of Wisconsin-Madison
A few weeks before Justin Bieber and his wife, Hailey, announced in May 2024 that they were expecting, the pop icon posted a selfie where he appears tearful and distraught.
While media attention quickly pivoted to the pregnancy, there was little attention paid to the significance of a male celebrity and expectant father publicly sharing his vulnerability.
Yet Bieber’s social media post is notable for making his internal struggle visible.
Emotional pain is linked to serious health issues. But the public’s response to male expressions of emotion and vulnerability is often minimizing, if not dismissive. In response to Bieber’s tearful post, for example, Hailey described him as a “pretty crier.”
A year ago, the Canadian rapper Dax released the song “To Be a Man.” He said at the time: “This is a song I poured my heart into. I’m praying this reaches everyone who needs it.”
Today, the song’s message remains timely. It includes the lyrics:
Yeah, I know this life can really beat you down, uh
You wanna scream but you won’t make a sound, uh
Got so much weight that you’ve been holdin’
But won’t show any emotion, as a man, that goes unspoken
As researchers who study fatherhood and the roles that men play in their families, we recognize the loneliness and pain in these lyrics. We have heard fathers describe the toll of attempting to keep a lid on their feelings.
In a recent study we conducted on 75 new and expectant Black fathers, they spoke of the need to address individual and collective trauma. This, they said, would ultimately help support their families. But they said resources to help men with their mental health are often unavailable or very limited. They said they often feel invisible to health providers.
“Being a father and a man,” one participant said, “you have to keep the peace and be strong on the outside. But on the inside, you know, you’re falling apart.”
Dax’s lyrics and our research reflect an enduring social health challenge – the deafening silence that typically surrounds men’s mental health.
The toll of isolation on men
In May 2023, U.S. Surgeon General Dr. Vivek Murthy released an advisory highlighting what he described as an epidemic of loneliness and isolation in the country. Our research confirms this scourge.
Since men’s social support networks – colleagues, family, close childhood friends – are often less robust than women’s, the epidemic disproportionately impacts men. The resulting solitude has very real health consequences.
In Murthy’s report, loneliness is associated with negative health outcomes, including a “29% increased risk of heart disease, a 32% increased risk of stroke, and a 50% increased risk of developing dementia for older adults. Additionally, lacking social connection increases risk of premature death by more than 60%.”
While Murthy’s report focuses on both men and women, research shows that men are less likely than women to seek mental health services. Additionally, men hold more negative attitudes toward seeking help, and they prematurely terminate treatment more often than women.
With these consequences in mind, a caring society may ask: Why are men carrying the brunt of this health risk, and what can be done about it?
Redefining men’s value beyond breadwinning
Many factors can contribute to feelings of isolation and disconnection among men.
In “To Be A Man,” Dax points toward one prominent factor:
As a man, we gotta pave our way
Our only function is to work and slave
There’s no respect for you if you ain’t paid
You’re disregarded as a human and you can’t complain
Traditional definitions of masculinity emphasize the importance of men’s role as breadwinners.
An uncertain economy and increasingly expensive housing and food prices make the ability to financially provide for a family elusive for many men. These factors also undermine men’s sense of self and contribute to loneliness and feelings of isolation.
As partners and fathers, men are still often perceived as deficient if they can’t provide economically. And societal norms stress that they are not valued for their capacity as caregivers, even if they are more involved in raising their children than ever before.
This is out of touch with reality.
Men play an important role as caregivers in their children’s lives, according to our research, and exert a powerful influence on children’s health and well-being. Men also find meaning in their roles as fathers.
As Dax says:
As a man, our son is our horizon
The cost of suppressed vulnerability
Beyond pressures to provide, men also have to overcome enduring stereotypes that suggest they should be stoic and keep their fears and sadness to themselves.
Here, too, gender norms are in need of an update. Boys and men need to feel comfortable presenting their true, authentic selves to the world. When they suppress their vulnerability, it creates a barrier to seeking help. It also perpetuates stigma and the epidemic of loneliness.
There is a complex interplay between society’s assumptions and beliefs about men and fatherhood.
Men, consequently, are less likely than women to seek mental health services. Health providers, as a result, are more likely to underdiagnose and misdiagnose men. Additionally, when health resources are made available, they are often not tailored to men’s needs.
Societal expectations can create unbearable pressure for men. And the most marginalized groups, like low-income Black fathers, bear a disproportionate burden, research shows. This became more evident during the COVID-19 pandemic, when Black fathers working in high-risk and essential jobs prioritized supporting their children and families over their own risk of infection and mental health.
As men continue to redefine their roles within families and communities, it’s important for society to create a space that acknowledges and embraces their vulnerabilities and full humanity in all social roles.
Men need outlets for their pain. They would benefit from relationships – with partners, family and friends – that support and nurture them in times of joy and through the emotional challenges. Their loneliness will continue to be disproportionate without the necessary connection to services.
Men can consider engaging in low-stakes discussion groups in their communities, with online groups and in their churches. They may also seek out therapists in person or online for introductory sessions to test out the therapeutic interaction before establishing a more consistent pattern of therapy services.
In “To Be a Man,” Dax sings:
No wonder most men are so depressed
All the things that they can’t express
It’s the circle of life, as a man, you provide
They don’t know what you’re worth ‘til the day that you die
As the Biebers adjust to life as parents, Justin may find people he can talk to about his experiences and emotions, people who see and value him fully. And we hope the same for every man and father, living their life out of the spotlight and doing the best they can for themselves and for their family.
Alvin Thomas, Associate Professor, Phyllis Northway Faculty Fellow, University of Wisconsin-Madison and Quinn Kinzer, Graduate student and PhD Candidate, Department of Consumer Science, University of Wisconsin-Madison
This article is republished from The Conversation under a Creative Commons license. Read the original article.
Our Lifestyle section on STM Daily News is a hub of inspiration and practical information, offering a range of articles that touch on various aspects of daily life. From tips on family finances to guides for maintaining health and wellness, we strive to empower our readers with knowledge and resources to enhance their lifestyles. Whether you’re seeking outdoor activity ideas, fashion trends, or travel recommendations, our lifestyle section has got you covered. Visit us today at https://stmdailynews.com/category/lifestyle/ and embark on a journey of discovery and self-improvement.
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Keep Cold and Flu Season at Bay
During cold and flu season, preparation and proper treatment are crucial for recovery, minimizing illness spread, and maintaining overall health.
(Family Features) It doesn’t take much for cold and flu symptoms to take over. Even a mild case of the sniffles can make it difficult to navigate the demands of everyday life. It’s virtually impossible to avoid germs altogether, but you can make sure you’re ready to fight off an infection, whether it’s a minor cold or a full-blown case of the flu.
Cold and Flu Tips
“I always share a few easy and simple methods with my patients when cold and flu season comes back around to keep them in the best shape,” said Dr. Tim Tiutan, board-certified internal medicine physician. “Not only does getting ahead offer protection, but knowing what to do when symptoms start to come on, and what to have in your medicine cabinet, can help ease the burden of an oncoming illness.”
Preparation and prevention play important roles before a bug hits, but it’s also valuable to know how to effectively treat an illness and keep your loved ones from getting sick.
Prevent
Short of stashing yourself away in a bubble, there aren’t many failsafe ways to fully avoid germs. However, some basic hygiene and preventive care can go a long way toward minimizing your risk. Regularly washing your hands can help get rid of germs before you accidentally introduce them to your eyes, mouth or nose. Be especially diligent after spending time in crowded places.
Maintaining an overall healthy lifestyle also helps ensure you’re in optimal condition if you do get sick. That means eating a nutritious, well-rounded diet and keeping up with your exercise routine. Also be sure to talk to your doctor or pharmacist about getting a flu shot; while it may not fully prevent you from getting sick, in most cases it helps your body fight back and keeps your symptoms manageable.
Prepare
Knowing you can’t completely eliminate your exposure to germs, it’s a good idea to ensure you have everything you need to fight a cold or flu on hand. At least once a year, give your medicine cabinet a thorough cleaning. Discard any expired medications and replace products that have run low, especially those designed to help fight symptoms like pain relievers, fever reducers, decongestants, antihistamines and cough syrups.
It’s also a good time to restock items like tissues, cough drops, hand sanitizer and antibacterial soap. Medical supplies like thermometers and humidifiers also come in handy when you’re under the weather; check to be sure yours are in good working order and consider adding any supplies you don’t already have, such as a blood pressure cuff or home oximeter.
Treat
Once you’re sick, sleep is one of the best remedies. Your body can redirect energy toward healing when you’re sleeping, which helps boost your immune system, so give yourself permission to rest when you’re feeling down.
In addition to getting enough rest, it’s important to treat your symptoms, not just mask them. Because symptoms like fever, runny nose, chest congestion, cough and more can indicate a more serious problem, finding the root cause of the symptoms can be a more effective treatment strategy.
For example, some common remedies for chest congestion only mask the symptoms. Many doctors recommend an option like Mucinex 12-Hour because it treats the cause of chest congestion by thinning and loosening excess mucus. One dose lasts up to 12 hours, helping you clear mucus and relieve chest congestion.
Contain
Getting rest and managing your symptoms are easier when you stay at home, but it’s also the best way to prevent spreading germs to others. It’s not always practical, but if you can, limit yourself to one area of the house, away from family members. Cough into your elbow or a tissue, never your hands. Use antibacterial hand sanitizer and wipe down surfaces to minimize others’ contact with your germs.
Be especially wary of spending time with anyone who has a compromised immune system while you’re contagious, including those with pre-existing conditions, older adults and pregnant women. Take advantage of delivery or curbside services for essentials like groceries and talk with your employer about working remotely or using sick time until you’re feeling better.
Find more advice to help fight this cold and flu season at Mucinex.com. https://www.youtube.com/embed/zUpFn6VhRTM?feature=share/?utm_source=familyfeatures&utm_medium=referral&utm_campaign=#17187-Mucinex
When to Call for Help if You’re Feeling Lousy
If you’re experiencing cold symptoms, you might wonder when it’s appropriate to see a doctor for further evaluation. While most colds resolve on their own within a week or two, there are certain instances when seeking medical attention is advisable.
High Fever: If your fever persists for more than three days or reaches 102 F or higher, consult a health care provider.
Persistent or Severe Headaches: If you experience persistent or severe headaches that are not alleviated by over-the-counter medication, it’s advisable to consult a health care provider, as this could indicate a more serious underlying issue.
Persistent Symptoms: If your symptoms worsen or don’t improve after a week, seek medical advice.
Unusual Symptoms: If you experience uncommon or concerning symptoms during a cold, such as extreme fatigue, confusion, dehydration or a high fever that doesn’t respond to treatment, consult your doctor promptly for a proper diagnosis and treatment plan.
Trouble Breathing: Difficulty breathing, chest pain or wheezing can indicate a more severe respiratory condition and warrant medical attention.
High-Risk Individuals: For individuals at high risk of complications from colds, such as pregnant women, the elderly or individuals with conditions like diabetes or heart disease, it’s important to seek advice early to prevent any potential complications.
Remember, your health care provider can offer guidance tailored to your specific situation, helping you manage your cold effectively and ensure optimal recovery.
Photos courtesy of Shutterstock
SOURCE:
Mucinex
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